Chris J. Frangieh

Perturb-ME combines CRISPR screening and single-cell profiling to identify regulatory modules governing gene programs in melanoma.

Compressed Perturb-seq reduces cost and complexity of CRISPR screens by using random composite perturbations and computational decomposition, achieving similar accuracy to conventional methods with greater power to detect genetic interactions.

The paper benchmarks a new low-cost sequencing by synthesis method for scRNA-seq, showing comparable results to existing technologies and enabling cost-effective large-scale applications.

The paper uses Perturb-CITE-Seq to identify novel immune evasion mechanisms in cancer by analyzing multi-modal single-cell data from patient-derived models.