Author

Marian A Pulgar

University of FloridaORCID 0009-0007-8191-200XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:yeast· 2 Research Focus:drug response / drug discovery· 1 Research Focus:protein structure prediction· 1 Assay:proteomics· 1

Frequent Co-Authors

Carl A. DenardiD· 2 shared Cassidy F Simas· 2 shared Ethan W SlatoniD· 2 shared Julia T Besu· 2 shared Sage E NelsoniD· 2 shared Samantha G. MartinuseniD· 2 shared Amit Kumar DuttaiD· 1 shared Seyednima Ajayebi· 1 shared

Activity by Year

2024-2025 · peak 1/yr

Co-Author Collaboration Network

9 top collaborators · 36 mutual co-author links

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2 shared papersSynthetic Biology

Carl A. DenardORCID

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2 papers

Recent·Most cited

Synthetic BiologyACS Chemical BiologyPeer-reviewedSep 23, 2025

High-throughput Activity Reprogramming of Proteases (HARP)

Samantha G. Martinusen, Ethan W Slaton, Seyednima Ajayebi…·Carl A. Denard

The paper introduces HARP, a yeast-based platform for discovering protease inhibitors, and demonstrates its ability to isolate low-nanomolar-range nanobodies against specific proteases.

3 citations

yeastproteomics

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Synthetic BiologyProtein Engineering Design and SelectionPeer-reviewedJan 1, 2024

Modular and integrative activity reporters enhance biochemical studies in the yeast ER.

Samantha G. Martinusen, Ethan W Slaton, Sage E Nelson…·Carl A. Denard

This paper expands the YESS system by creating integrative plasmids for modular and uniform activity reporting of PTM-enzymes in yeast.

4 citations

yeastexperimental assay

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