High-throughput Activity Reprogramming of Proteases (HARP)
Samantha G. Martinusen, Ethan W Slaton, Seyednima Ajayebi, Marian A Pulgar, Cassidy F Simas, Sage E Nelson, Amit Kumar Dutta, Julia T Besu, Steven D. Bruner, Carl A. Denard
The paper introduces HARP, a yeast-based platform for discovering protease inhibitors, and demonstrates its ability to isolate low-nanomolar-range nanobodies against specific proteases.
ACS Chemical Biology · Sep 23, 2025Peer-reviewedSynthetic Biology