In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

B. Kakuk; A. Dormo; Z. Csabai; G. Kemenesi; J. Holoubek; D. Ruzek; I. Prazsak; V. E. Dani; B. Denes; G. Torma; F. Jakab; G. E. Toth; F. V. Foldes; B. Zana; Z. Lanszki; A. Harangozo; A. Fulop; G. Gulyas; M. Mizik; A. A. Kiss; D. Tombacz; Z. Boldogkoi

doi:10.1038/s41597-023-02149-4

MicrobiologyScientific DataMay 9, 2023

Peer-reviewed journal article

In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

B. Kakuk, A. Dormo, Z. Csabai, G. Kemenesi, J. Holoubek, D. Ruzek, I. Prazsak, V. E. Dani, B. Denes, G. Torma, F. Jakab, G. E. Toth, F. V. Foldes, B. Zana, Z. Lanszki, A. Harangozo, A. Fulop, G. Gulyas, M. Mizik, A. A. Kiss, D. Tombacz, Z. Boldogkoi

Abstract

The recent Monkeypox outbreak showed the importance of studying the basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. Our direct cDNA and native RNA sequencing data enable the in-depth characterization of the transcriptomic architecture and dynamics of the gene expressions of monkeypox virus; and also the deeper understanding of the changes it causes in the host cells on a transcriptome level.

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