Advanced neural activity mapping in brain organoids via field potential imaging with ultra-high-density CMOS microelectrodes.

Abstract

Human iPSC-derived brain organoids and assembloids have emerged as promising in vitro models for recapitulating human brain development, neurological disorders, and drug responses. However, detailed analysis of their electrophysiological properties requires advanced measurement techniques. Here, we present a novel analytical approach utilizing ultra-high-density (UHD) CMOS microelectrode arrays (MEAs) containing 236,880 electrodes (10.52 m x 10.52 m each) distributed over a broad sensing area of 32.45 mm2 for field potential imaging (FPI) of brain organoids. Neuronal activity was recorded simultaneously from over 46,000 electrodes interfaced with brain organoids, allowing for the identification of single-cell firing events and the assessment of neuronal network connectivity based on individual spikes. In midbrain organoids, administration of L-DOPA revealed both excitatory and inhibitory cellular responses, with a dose-dependent increase in the proportion of excitatory responses, suggesting enhanced network connectivity. Capitalizing on the spatial and temporal resolution of UHD-CMOS-MEAs, we introduced new endpoints for network activity: propagation velocity and propagation area. In cortical organoids, application of the GABAA receptor antagonist picrotoxin led to increased propagation velocity, whereas the NMDA receptor antagonist MK-801 resulted in a broad reduction of propagation area, along with localized increases. As FPI enables direct recording of electrical potential waveforms, frequency-domain analyses were also conducted. Spontaneous activity in cortical organoids exhibited region-specific frequency distributions, with gamma-band activity displaying distinct patterns compared to other frequency bands. Additionally, in midbrain-striatal assembloids, electrophysiological activity was observed in both regions. Connectivity analysis showed that treatment with 4-aminopyridine enhanced inter-organoid connection strength. This large-scale, single-cell-resolved recording approach using UHD-CMOS-MEAs facilitates comprehensive analysis of network connectivity, propagation velocity and propagation area, and frequency characteristics. It represents a powerful platform for advancing our understanding of the electrophysiological functions of brain organoids and assembloids, and holds significant potential for drug screening and disease modeling in human neuroscience research.