Tagging fluorescent reporter to epinecidin-1 antimicrobial peptide

In this study, we successfully cloned the fluorescent proteins eGFP and DsRed in-frame with the antimicrobial peptide epinecidin-1 (FIFHIIKGLFHAGKMIHGLV). The cloning strategy involved inserting the fluorescent reporters into the expression vector, followed by screening of positive clones through visual fluorescence detection and molecular validation. The visually identified fluorescent colonies were found positive with PCR and plasmid migration assay confirming successful cloning. This fusion of fluorescent reporters with a short antimicrobial peptide enables real-time visualization and monitoring of the peptides mechanism of action on membranes and within cells, both in vivo and in vitro. The fusion of eGFP and DsRed to epinecidin-1 did not impair the expression or fluorescence of the reporter protein.