Structural bases for blockade and activation of BK channels by Ba2+ ions
S. Srivastava, P. Miranda Fernandez, N. de Val, T. Giraldez, J. Zhu, R. E. Cachau, M. Holmgren
Abstract
Ba2+ increases open probability and blocks the permeation pathway of BK channels. Here we discuss Cryo-EM structures of BK channels with Ba2+. They reveal that Ba2+ occupies site S3 in the selectivity filter, leading to blockade. Ba2+ are also detected at all high affinity Ca2+ binding sites of the gating ring. However, the architectures of this region in these structures represent intermediate transitions between the extreme close and open configurations.
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