ThirdPeak: A flexible tool designed for the robust analysis of two- and three-dimensional (single-molecule) tracking data.

Abstract

ThirdPeak is an open-source tool tailored for the comprehensive analysis of two- and three-dimensional track data across various scales. Its versatile import options facilitate seamless integration into established workflows, and the user interface allows for swift visualization and analysis of the data. When applied to live-cell diffusion data, this software unveils the advantages of combining both 2D and 3D analysis, providing valuable insights into the understanding of biological processes.