Calibration-free molecular counting from a single DNA-PAINT intensity trace using cumulants
Teun A.P.M. Huijben, Rodolphe Marie, Jonas Nyvold Pedersen
This paper introduces mCOAST, a calibration-free method for accurate molecular counting from DNA-PAINT intensity traces without requiring prior knowledge or extensive post-processing.
bioRxiv · Nov 17, 2025 · PreprintBiophysics