Christian Eggeling

K-Ras controls asymmetric cell divisions from the primary cilium

This study shows K-Ras4B sustains ciliation during muscle cell differentiation by localizing to the primary cilium, affecting asymmetric cell divisions and differentiation outcomes.

Overcoming Protein A-driven Nonspecific Antibody Staining of S. aureus in Immunofluorescence Microscopy

The paper evaluates methods to reduce nonspecific antibody staining caused by Staphylococcus aureus protein A in immunofluorescence microscopy, finding human serum treatment most effective.

Parameter Optimization for Iterative MINFLUX Microscopy enabled Single Particle Tracking

The paper introduces a parameter optimization strategy for iterative MINFLUX microscopy to improve single particle tracking of fast lipid diffusion in membranes.

NanTex enables computational multiplexing and phenotyping of organelles across super-resolution modalities

NanTex enables multiplexing and phenotyping of organelles using nanotexture descriptors from single-channel super-resolution microscopy data across multiple modalities.

Event-triggered MINFLUX microscopy: smart microscopy to catch and follow rare events

The paper introduces event-triggered MINFLUX microscopy to efficiently capture rare cellular events by triggering MINFLUX acquisition based on real-time confocal imaging analysis.

CCB79 is a primate-specific cilium initiation factor essential to maintain neural progenitor diversity in developing brain tissue.

This study identifies CCB79 as a primate-specific protein essential for maintaining neural progenitor diversity in developing brains by regulating ciliogenesis.

Spectral STED microscopy improves spectral sensitivity with polarity-sensitive probes and enables correlative measurements of membrane order and anomalous lipid diffusion

Homogeneous large field-of-view and compact iSCAT-TIRF setup for dynamic single molecule measurements

The paper introduces a compact iSCAT-TIRF setup for dynamic single molecule measurements with a large field-of-view and uniform illumination.

TRAIT2D: a Software for Quantitative Analysis of Single Particle Diffusion Data

TRAIT2D is a Python software for analyzing single particle diffusion data, offering tracking, analysis, and simulation with a graphical interface.

Addressing differentiation in live human keratinocytes by assessment of membrane packing order

This study develops a live-cell method to assess keratinocyte differentiation using membrane packing order as a marker, applicable at single-cell and high-throughput levels.

Object Detection Networks and Augmented Reality for Cellular Detection in Fluorescence Microscopy Acquisition and Analysis.

This paper applies object detection networks to classify and localize cells in fluorescence microscopy, demonstrating effectiveness with small datasets and integrating an AR system for real-time imaging.

Advanced processing and analysis of conventional confocal microscopy generated scanning FCS data

This paper optimizes processing and analysis of scanning FCS data, introducing FoCuS-scan software and addressing bias and variance in diffusion measurements.

Laurdan and di-4-ANEPPDHQ probe different properties of the membrane

This study compares Laurdan and di-4-ANEPPDHQ probes to determine if they measure the same membrane properties, finding they report different lipid packing characteristics.

Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS

The paper measures diffusion dynamics of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles using STED-FCS, showing actin cortex influences diffusion modes.