M. Engstler

Quantification of Trypanosoma Brucei social motilityindicates different colony growth phases

This study quantifies social motility in Trypanosoma brucei colonies using scale-free metrics and identifies two distinct growth phases.

The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei

This study investigates the role of the actomyosin system in maintaining endosomal integrity in Trypanosoma brucei using imaging and biochemical assays.

ThirdPeak: A flexible tool designed for the robust analysis of two- and three-dimensional (single-molecule) tracking data.

ThirdPeak analyzes 2D and 3D single-molecule tracking data, revealing insights from combined 2D and 3D analysis of live-cell diffusion.

The dual role of the 16mer motif within the 3' untranslated region of the variant surface glycoprotein of Trypanosoma brucei

This study investigates the dual role of a conserved 16mer motif in the 3' UTR of Trypanosoma brucei VSG genes, showing it is essential for transcript stability but not required for VSG silencing or differentiation.

Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes

The paper investigates the endosomal system in Trypanosoma brucei, revealing it as a continuous membrane system rather than distinct compartments, enabling rapid membrane trafficking.

The intracellular amastigote of Trypanosoma cruzi maintains an actively beating flagellum

This study shows that intracellular Trypanosoma cruzi amastigotes have a beating flagellum, challenging the previous belief that these forms are non-motile.

Single-molecule fluorescence microscopy demonstrates fast dynamics of the variant surface glycoprotein coat on living trypanosomes

The paper uses single-molecule tracking to show that VSG coat dynamics on trypanosomes are primarily diffusive with a high diffusion coefficient, resolving how the parasite maintains its coat.

The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei

This study identifies Hel66, a DExD/H box helicase, as essential for ribosome biogenesis in Trypanosoma brucei, showing its role in translation and rRNA processing.

Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms

This study tracks Trypanosoma brucei development in artificial human skin, identifying a quiescent state called skin tissue forms that may sustain infection.

Endoplasmic reticulum-targeting but not translation is required for mRNA balancing in trypanosomes

This study shows that ER targeting, not translation, regulates VSG mRNA levels in trypanosomes, suggesting ER feedback controls mRNA amounts to prevent secretory pathway overload.

Nanobody Mediated Macromolecular Crowding Induces Membrane Fission and Remodeling in the African Trypanosome

The paper shows that nanobodies induce membrane fission and remodeling in African trypanosomes by causing macromolecular crowding, leading to loss of motility and nanovesicle shedding.

Unexpected plasticity in the life cycle of Trypanosoma brucei

This study shows that proliferating slender stage Trypanosoma brucei can infect tsetse flies as effectively as stumpy stages, challenging the traditional view of the parasite's life cycle.

18S rDNA Sequence-Structure Phylogeny of the Trypanosomatida (Kinetoplastea, Euglenozoa) with Special Reference on Trypanosoma

The paper uses 18S rDNA sequence and secondary structure data to reconstruct a robust phylogeny of trypanosomatids, showing improved resolution compared to sequence-only analyses.

Parallel monitoring of mRNA abundance, localisation and compactness with correlative single molecule FISH on LR White embedded samples

The paper introduces LR White smFISH to monitor mRNA abundance, localisation, and compactness in dense structures, using trypanosomes as a model system.

Positional dynamics and glycosomal recruitment of developmental regulators during trypanosome differentiation

The paper tracks TbPIP39 recruitment to glycosomes during trypanosome differentiation and identifies a regulatory site coordinating signaling and glycosomal development.