S. Holden

Complement-mediated killing of bacteria by mechanical destabilization of the cell envelope

The paper uses atomic force microscopy to study how MAC pores in E. coli lead to mechanical destabilization of the cell envelope, causing lysis through inner membrane permeation.

Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth

This study shows that transertion and cell geometry influence the spatial organization of the E. coli nucleoid during growth and antibiotic treatment.

A one-track model for spatiotemporal coordination of Bacillus subtilis septal cell wall synthesis

This study uses live-cell imaging to show that PBP2B moves processively and asynchronously with FtsZ in B. subtilis, supporting a new model of septal cell wall synthesis.

Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis

This study tracks elongasome dynamics in Bacillus subtilis using single molecule fluorescence microscopy and shows molecular motor tug-of-war regulates processivity and termination events.

Self-organisation of mortal filaments: the role of FtsZ treadmilling in bacterial division ring formation

The paper shows that FtsZ treadmilling drives nematic ordering in bacterial cells, enabling division ring formation through local dissolution of misaligned filaments.

Whole genome sequencing suggests that non-pathogenicity on banana (NPB) is the ancestral state of the Ralstonia solanacearum IIB-4 lineage.

A lineage-specific Exo70 is required for receptor kinase-mediated immunity in barley

The paper identifies a lineage-specific Exo70FX12 gene required for Rps8-mediated immunity in barley against wheat stripe rust.

DeepBacs: Bacterial image analysis using open-source deep learning approaches

DeepBacs uses open-source deep learning to analyze bacterial microscopy images, offering tools for segmentation, classification, and phenotyping with applications in antibiotic research.

Enhanced cGAS-STING-dependent interferon signaling associated with mutations in ATAD3A

The paper identifies mutations in ATAD3A causing enhanced type I interferon signaling in patients with neurological and autoimmune features.

Genetic variation, environment and demography intersect to shape Arabidopsis defense metabolite variation across Europe

The paper examines how genetic variation, environment, and demography influence defense metabolite variation in Arabidopsis thaliana across Europe.

Geometric principles underlying the proliferation of a model cell system

This study investigates how cell geometry affects growth, chromosome segregation, and division in Bacillus subtilis L-forms, showing that surface area over volume and nucleoid positioning are key factors.

FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell division

Virtual-SMLM, a virtual environment for real-time interactive SMLM acquisition

Virtual-SMLM provides a virtual environment for real-time interactive single molecule localisation microscopy acquisition, enabling training and validation of automated methods with realistic simulated data.

Super-resolution fight club: A broad assessment of 2D & 3D single-molecule localization microscopy software

The paper evaluates 36 single-molecule localization microscopy software packages using simulated datasets to assess their performance in 2D and 3D imaging.

Movement Dynamics of Divisome and Penicillin-Binding Proteins (PBPs) in Cells of Streptococcus pneumoniae

This paper investigates FtsZ dynamics and its relationship to septal PG synthesis in Streptococcus pneumoniae, finding that FtsZ movement is continuous and independent of PG synthesis rate.

Constriction rate modulation can drive cell size control and homeostasis in C. crescentus

This study shows that constriction rate in C. crescentus affects cell size control and homeostasis by regulating elongation during division and compensating for pre-constriction variation.