bioRxivGenomicsPreprint
Records of RNA localization through covalent tagging
Hugo C. Medina-MuñoziD, Christopher P. LapointeiD, Douglas F. PorteriD, Marvin WickensiD
Abstract
RNA movements and localization pervade biology, from embryonic development to disease. To identify RNAs at specific subcellular locations, we anchored a uridine-adding enzyme at those sites, which then marked RNAs in its vicinity with 3 terminal uridines. RNAs were tagged independent of their translation status, and included not only mRNAs, but also ncRNAs and ncRNA processing intermediates. A battery of RNAs, including the stress sensor, IRE1, were tagged at both ER and mitochondria, and reveal RNAs whose dual localization is conserved from yeast to human cells.
- organism: yeast
- organism: human
- assay: bulk RNA-seq
- research focus: cell type annotation
- approach: experimental assay
- Citations
- 3
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