Alice Y. Ting

This paper designs a new proximity labeling enzyme, FlexID, using computational methods to improve spatial precision and versatility for mapping protein interactions and proteomes.

This paper introduces a cell-based sensor called LUNAR that detects extracellular double-stranded DNA with nanomolar sensitivity in mammalian cells.

This paper introduces LATeNT, a light-activated tetanus neurotoxin that enables conditional proteolysis and inducible synaptic inhibition in vivo, with applications in neuroscience and beta cell function.

This paper introduces SynPARs, synthetic protease-activated receptors that enable cells to sense and respond to protease activity, demonstrating their use in counteracting hyperalgesia in mice.

The paper maps the Drosophila secretome to identify tissue-specific origins and inter-organ trafficking of 535 blood plasma proteins.

This paper introduces scAPEX-seq, a method to map subcellular transcriptomes, and identifies CTSW as a regulator of CAR T cell function in tumor co-cultures.

The paper introduces endocytome profiling to map cell-surface protein dynamics in developing olfactory receptor neurons, revealing how endocytosis regulates axon pruning and targeting.

This paper develops a method to edit mitochondrial DNA in yeast using CRISPR by identifying an RNA import motif that enables sgRNA delivery into mitochondria.

Contrastive scoring of protein variants across alternative backbone structures with inverse folding models (Conformational Biasing) predicts mutations that shift conformational equilibria and tune effector binding, catalysis, and labeling specificity.

The paper characterises LAMP1- and LAMP2A-positive organelles in neurons, finding they have distinct transport dynamics and protein compositions.

This study evolves a laccase enzyme for cell surface labeling and EM, showing it can map T cell surface changes and visualize cell features without toxic H2O2.

The paper develops a covalent protein-RNA conjugation method using a modified HUH tag through directed evolution, achieving high sensitivity and efficiency for RNA labeling.

The paper introduces synthetic GPCRs that can be programmed to control cell behavior in response to specific antigens, using modular design for versatile applications.

The paper identifies hundreds of Connexin-associated proteins in zebrafish electrical synapses, revealing molecular diversity and overlap with chemical synapse proteomes, and links these proteins to neurological disorders.

This study identifies the intracellular signaling pathway of teneurins in synaptic partner matching using Drosophila models.

The paper describes the development of genetically encodable LYTACs (GELYTACs) for targeted protein degradation using engineered T-cells, showing efficacy in secreting and delivering the therapeutics.

This paper introduces LOV-Turbo, a light-regulated proximity labeling tool that enhances spatial and temporal control of proteomic mapping in living cells.

This study identifies Acj6 as a transcription factor that controls dendrite targeting in Drosophila PNs by regulating combinatorial expression of cell-surface proteins.

This paper introduces LuCID, a tool that detects calcium in real-time and converts it into stable gene expression, with applications beyond calcium signaling.

The paper introduces APEX-PS to map RNA binding proteins in cellular compartments, identifying SYNJ2BP as a mitochondrial mRNA anchor that aids stress recovery.

The paper develops and benchmarks in vivo TurboID and miniTurbo labeling in zebrafish embryos for proximity-dependent biotinylation, enabling protein discovery in living vertebrates.

The paper introduces FLiCRE, a molecular tool for tagging calcium-activated cells, and uses it to identify and manipulate a striatal cell-type involved in aversion behavior.

This study maps RNA-protein interactions in living cells using MS2 and Cas13-based APEX2 targeting, identifying novel interactions like hTR and ALKBH5.

The paper profiles cell-surface proteomes in Drosophila olfactory neurons across development, identifying new regulators of neural circuit assembly.

The paper evolves TEV protease to improve its catalytic efficiency using a yeast-based directed evolution platform with light-regulated selection.

This study describes the directed evolution of a split APEX peroxidase, demonstrating its reconstitution and functionality in different cellular contexts.