Chromosome-level genome assembly of the gerbera (Gerbera hybrida) using HiFi long-read and Hi-C technologies
Yuta Aoyagi BlueiD, Riki Shimada, Hideki HirakawaiD, Atsushi ToyodaiD, Hidehiro TohiD, Sachiko IsobeiD, Naoyuki TajimaiD, Kenta ShirasawaiD, Tokumasa HoriikeiD, Hiroshi FujiiiD, Tao FujiwaraiD, Masaru BambaiD, Takashi Nakatsuka, Akiyoshi Tominaga
Abstract
Gerbera hybrida is one of the most popular ornamental plants and also serves as a valuable model plant within the Asteraceae family. Here, we report both the nuclear and organellar genome assemblies and annotations of G. hybrida, which was developed through hybridization of two wild species. Sequencing was performed using a combination of PacBio high fidelity (HiFi) reads and chromatin capture reads (Omni-C). The total span of the nuclear genome assembly is 2.32 gigabases, and 99.3% of the sequence assembled into 25 scaffolds, consistent with the known chromosome number. Genome annotation of the nuclear genome identified 36,160 protein-coding genes and 11,572 non-coding transcripts. The mitochondrial genome had 363,511 bp and contains 36 protein-coding genes, 3 rRNAs, and 21 tRNAs, while the chloroplast genome is 151,898 bp in length and includes 85 protein-coding genes, 8 rRNAs, and 37 tRNAs. This reference genome provides a foundational resource for future molecular breeding and genetic research in Gerbera and the broader Asteraceae family.
02 /Specification & Classification
Click any tag to browse matching papers- Citations
- 2
- FWCI
- 7.36
- Field percentile
- top 5%
- Organism
- Assay
- Research Focus
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Chromosome-level Genome Assembly and Annotation of Petunia hybrida
Ali Saei, Donald Alexander Hunter, … David Chagné·bioRxiv·Sep 19, 2024
- Genome assembly of the hybrid grapevine Vitis Chambourcin
Sagar S. Patel, Zachary N. Harris, … Anne Y. Fennell·GigabytePeer-reviewed·Jul 3, 2023
- Bi-parental graph strategy to represent and analyze hybrid plant genomes
Qianqian Kong, Yi Jiang, … Junpeng Shi·PLANT PHYSIOLOGYPeer-reviewed·Jul 11, 2024
- HiFi chromosome-scale diploid assemblies of the grape rootstocks 110R, Kober 5BB, and 101-14 Mgt
Andrea Minio, Noé Cochetel, … Dario Cantù·Scientific DataPeer-reviewed·Oct 28, 2022
- Chromosome-scale genome assembly of Eustoma grandiflorum, the first complete genome sequence in family Gentianaceae
Kenta Shirasawa, Ryohei Arimoto, … Sachiko Isobe·bioRxiv·Sep 9, 2021
- A chromosome-level genome assembly of amodel conifer plant, the Japanese cedar,Cryptomeria japonica D. Don
Takeshi Fujino, Katsushi Yamaguchi, … Masahiro Kasahara·BMC GenomicsPeer-reviewed·Nov 5, 2024