Diverse noncanonical PAMs recognized by SpCas9 in human cells
Z. Hu, D. Wang, C. Zhang, S. Wang, S. Gao, L. Hou, H. Wang, Y. Wang
Abstract
The CRISPR/Cas9 system derived from Streptococcus pyogenes (SpCas9) provides unprecedented genome editing capabilities, but the potential for off-target mutations limits its application. In addition to NGG protospacer adjacent motif (PAM), off-target mutations are also associated with noncanonical PAMs, which have not yet been systematically evaluated. Here, we developed a highly sensitive approach that allows systematically analyzing PAM sequences in human cells, and identified multiple alternative PAMs recognized by SpCas9.
02 /Specification & Classification
Click any tag to browse matching papers- Organism
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Divergent PAM Specificity of a Highly-Similar SpCas9 Ortholog
P. Chatterjee, N. Jakimo, J. M. Jacobson·bioRxiv·Feb 2, 2018
- Unraveling the mechanisms of PAMless DNA interrogation by SpRY Cas9
G. N. Hibshman, J. P. Bravo, … D. W. Taylor·bioRxiv·Jun 22, 2023
- Closely related type II-C Cas9 orthologs recognize diverse PAMs
Jingjing Wei, Linghui Hou, … Yongming Wang·eLifePeer-reviewed·Aug 12, 2022
- High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation
M. Legut, Z. Daniloski, … N. E. Sanjana·bioRxiv·Jan 22, 2020
- Reprogramming Cas9 PAM Recognition for Allele-Specific Editing
Julia Amber Tartaglia, Vivian Nguyen, … David F. Savage·bioRxiv·Aug 11, 2026
- A Cas9 with Complete PAM Recognition for Adenine Dinucleotides
N. Jakimo, P. Chatterjee, … J. M. Jacobson·bioRxiv·Sep 27, 2018