bioRxivGenomicsPreprint
The Hi-Culfite assay reveals relationships between chromatin contacts and DNA methylation state
Elena K. Stamenova, Neva C. DurandiD, Olga DudchenkoiD, Muhammad S. ShamimiD, Su‐Chen Huang, Yiqun Jiang, Ivan D. BochkoviD, Suhas S.P. RaoiD, Eric S. LanderiD, Andreas GnirkeiD, Erez Lieberman AideniD
Abstract
Hi-Culfite, a protocol combining Hi-C and whole-genome bisulfite sequencing (WGBS), determines chromatin contacts and DNA methylation simultaneously. Hi-Culfite also reveals relationships that cannot be seen when the two assays are performed separately. For instance, we show that loci associated with open chromatin exhibit context-sensitive methylation: when their spatial neighbors lie in closed chromatin, they are much more likely to be methylated.
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