Prospects of using Avocado oil for attenuating quorum sensing regulated virulence, bio-filming formation and its antibacterial and antioxidant activities

ABSTRCTQuorum sensing inhibition (QSI) is considered as an attractive strategy for the development of anti-pathogenic agents, mainly for drug resistant bacteria. The anti-quorum sensing activity was investigated by biosensor bioassay using Chromobacterium violaceum CVO26 and Pseudomonas aeruginosa PAO1. Quorum sensing is a key regulator of virulence factors of Pseudomonas aeruginosa such as bio-film formation, motility, productions of proteases, hemolysin, and Pyocyanin production. Additionally, the GC/MS technique was employed to detect the essential components of avocado oil. Avocado oil inhibits quorum system-mediated virulence factor production such as violacein in C. Violaceum CVO26 and elastase, Pyocyanin production in Pseudomonas aeruginosa PAO1. Additionally, the use of sub-minimum inhibitory concentrations (sub-MICs) of avocado oil significantly inhibits the quorum system-mediated biofilm formation, exopolysaccride production (EPS) and swarming motility. Furthermore, this study concerned the potent activity of avocado oil antibacterial and antioxidant agent. Moreover, a total of 23 components was identified in avocado oil by GC/MS.Avocado oil could be exploited as a natural source of anti-pathogenic, where the pathogenicity is mediated through quorum sensing, antibacterial as well as antioxidant agents.\n\nGraphical Abstract\n\nO_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=146 SRC=\"FIGDIR/small/486928_ufig1.gif\" ALT=\"Figure 1\">\nView larger version (30K):\norg.highwire.dtl.DTLVardef@8f44fborg.highwire.dtl.DTLVardef@1f9f371org.highwire.dtl.DTLVardef@ddc15aorg.highwire.dtl.DTLVardef@c136a0_HPS_FORMAT_FIGEXP M_FIG C_FIG