S.-S. Chng

Primary role of the Tol-Pal complex in bacterial outer membrane lipid homeostasis

The paper uncouples Tol-Pal complex function in maintaining outer membrane lipid homeostasis from its role in cell division in E. coli.

Deciphering the role of the OmpC-Mla system in bile salt resistance

This study identifies a novel role for the OmpC-Mla system in bile salt resistance in E. coli, independent of its known function in maintaining outer membrane lipid asymmetry.

Defining a critical role of an essential membrane protein in mycolic acid transport in mycobacteria

This study identifies MSMEG_0317 as essential for mycolic acid transport in Mycobacterium smegmatis, showing its role in maintaining the outer membrane structure.

Depleting cationic lipids involved in antimicrobial resistance drives adaptive lipid remodeling in Enterococcus faecalis

This study shows that depleting cationic lipids in Enterococcus faecalis leads to adaptive lipid remodeling, including reduced PG, increased phosphorus-containing lipids, and altered fatty acid metabolism.

Molecular mechanism of phospholipid transport at the bacterial outer membrane interface

This paper characterizes the MlaA-MlaC complex structure and mechanism for phospholipid transport across the bacterial outer membrane.

A conserved membrane protein negatively regulates Mce1 complexes in mycobacteria

The paper identifies a conserved membrane protein that negatively regulates Mce1 complexes in mycobacteria by inhibiting transporter assembly.

ATP disrupts lipid binding equilibrium to drive retrograde transport critical for bacterial outer membrane asymmetry

This paper reveals how ATP disrupts lipid binding equilibrium to drive retrograde phospholipid transport by the OmpC-Mla system, maintaining outer membrane asymmetry in Gram-negative bacteria.

Genetic interaction mapping highlights key roles of the Tol-Pal complex

The paper maps genetic interactions of the Tol-Pal complex in E. coli, revealing its roles in OM homeostasis, cell wall remodeling, and division.

Mutations in enterobacterial common antigen biosynthesis restore outer membrane barrier function in Escherichia coli tol-pal mutants

The paper identifies ECA biosynthesis mutations that restore OM barrier function in E. coli tol-pal mutants without restoring lipid homeostasis.

Structure-function characterization of the conserved regulatory mechanism of the Escherichia coli M48 metalloprotease BepA

The paper presents the crystal structure of E. coli BepA, an M48 metalloprotease, and shows that its active site plug regulates function and is conserved across the family.

Auranofin inhibits virulence in Pseudomonas aeruginosa

This study shows that auranofin inhibits virulence in Pseudomonas aeruginosa by targeting Vfr, reducing quorum sensing and Type IV pili, and synergizing with colistin to eliminate biofilms.

Structure-function analysis of the Escherichia coli β-barrel assembly enhancing protease BepA suggests a role for a self-inhibitory state

This study presents the crystal structure of BepA, a protease involved in LptD folding, and shows its auto-regulation and role in maintaining outer membrane lipid asymmetry.

MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis

The paper identifies and characterizes MCE domain proteins in E. coli, showing they localize to the inner membrane and play distinct roles in maintaining outer membrane lipid asymmetry.