CRISPRpic: Fast and precise analysis for CRISPR-induced mutations via prefixed index counting

Analysis of CRISPR-induced mutations at targeted loci can be achieved by PCR amplification followed by massively parallel sequencing. We developed a novel algorithm, called CRISPRpic, to analyze sequencing reads from CRISPR experiments via counting exact-matches and pattern-searching. Compared to other methods that are based on sequence alignment, CRISPRpic provides precise mutation calling and ultrafast analysis of sequencing results. The Python script for CRISPRpic is available at https://github.com/compbio/CRISPRpic.