Cross-talk between QseBC and PmrAB two-component systems is crucial for regulation of motility and colistin resistance in Enteropathogenic Escherichia coli

Abstract

The quorum sensing two-component system (TCS) QseBC has been linked to virulence, motility and metabolism regulation in multiple Gram-negative pathogens, including Enterohaemorrhagic Escherichia coli (EHEC), Uropathogenic E. coli (UPEC) and Salmonella enterica. In EHEC, the sensor histidine kinase (HK) QseC detects the quorum sensing signalling molecule AI-3 and also acts as an adrenergic sensor binding host epinephrine and norepinephrine. Downstream changes in gene expression are mediated by phosphorylation of its cognate response regulator (RR) QseB, and cross-talk with non-cognate regulators KdpE and QseF to activate motility and virulence. In UPEC, cross-talk between QseBC and TCS PmrAB is crucial in the regulation and phosphorylation of QseB RR that acts as a repressor of multiple pathways, including motility. Here, we investigated QseBC regulation of motility in the atypical Enteropathogenic E. coli (EPEC) strain O125ac:H6, causative agent of persistent diarrhoea in children, and its possible cross-talk with the KdpDE and PmrAB TCS. We showed that in EPEC QseB acts as a repressor of genes involved in motility, virulence and stress response, and in absence of QseC HK, QseB is activated by the non-cognate PmrB HK, similarly to UPEC. We show that in absence of QseC, phosphorylated QseB activates its own expression, and is responsible for the low motility phenotypes seen in a QseC deletion mutant. Furthermore, we showed that KdpD HK regulates motility in an independent manner to QseBC and through a third unidentified party different to its own response regulator KdpE. We showed that PmrAB has a role in iron adaptation independent to QseBC. Finally, we showed that QseB is the responsible for activation of colistin and polymyxin B resistance genes while PmrA RR acts by preventing QseB binding to the promoter of polymyxin resistance genes. Author summaryEnteropathogenic Escherichia coli (EPEC) is a human pathogen and the leading cause of diarrhoea in children under 5 years in low-income countries. EPEC, and specially the subgroup denominated atypical EPEC, has been associated with community-acquired persistent diarrhoea and also is an important agent of post-weaning diarrhoea in young piglets. Two-component systems (TCS) are signalling system used by bacteria to sense and adapt to different stimuli. Bacteria use TCS to detect host signals as queues to exert virulence in their preferred niche. QseBC is a TCS that has been linked to regulation of motility and virulence in other pathogenic E. coli. In this study we broaden our understanding of QseBC TCS and its role in regulating virulence traits such as motility in atypical EPEC. We also investigate its capabilities to interact with other TCS, named KdpDE and PmrAB, and how these interactions are responsible for regulation of motility and resistance to antimicrobials such as colistin.