Expanding TheCellMap.org to visualize a genome-scale genetic interaction network for a human cell line

Ira Horecka; Matej Ušaj; Myra Paz David Masinas; Henry N. Ward; Xiang Zhang; Arshia Zernab Hassan; Maximilian Billmann; Hannes Luc Röst; Chad L. Myers; Michael Costanzo; Brenda Andrews; Charles M. Boone

doi:10.64898/2026.03.16.712129

Systems BiologybioRxivMar 18, 2026

Preprint

Expanding TheCellMap.org to visualize a genome-scale genetic interaction network for a human cell line

Ira Horecka iD, Matej Ušaj iD, Myra Paz David Masinas, Henry N. Ward iD, Xiang Zhang iD, Arshia Zernab Hassan iD, Maximilian Billmann iD, Hannes Luc Röst iD, Chad L. Myers iD, Michael Costanzo iD, Brenda Andrews iD, Charles M. Boone iD

Abstract

Genetic interaction networks map functional connections between genes and their corresponding pathways and complexes. We previously developed TheCellMap.org as a central repository for storing and analyzing quantitative genetic interaction data produced by genome-scale Synthetic Genetic Array (SGA) analysis in the budding yeast, Saccharomyces cerevisiae. We have expanded TheCellMap.org to include ~89,000 quantitative genetic interactions identified from genome-scale CRISPR-based analysis of ~4 million human gene pairs in the haploid cell line, HAP1. TheCellMap.org enables users to readily access, visualize and explore human HAP1 genetic interactions, as well as to extract and reorganize sub-networks, applying data-driven network layouts in an intuitive and interactive manner.

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