Quantitative measurements of chromatin modification dynamics during zygotic genome activation
Y. Sato, L. Hilbert, H. Oda, Y. Wan, J. M. Heddleston, T.-L. Chew, V. Zaburdaev, P. Keller, T. Lionnet, N. Vastenhouw, H. Kimura
Abstract
Histone posttranslational modifications (PTMs) are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We describe an approach that images fluorescent antibody fragments to quantify PTM enrichment and active transcription at defined loci during zygotic genome activation in living zebrafish embryos. The technique reveals a drastic increase in histone H3 Lys27 acetylation (H3K27ac) before genome activation both at a specific locus and globally.
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