Timothy J. Stasevich

This study uses live-cell imaging to show SARS-CoV-2 Nsp1 silences mRNAs by reducing their numbers through two cap-dependent mechanisms, while viral UTRs enhance translation but do not protect from Nsp1.

This study uses real-time single-molecule imaging in zebrafish embryos to reveal non-canonical translation mechanisms of bmp2b mRNA.

The paper develops a cysteine-free UTag system for live-cell single-mRNA translation tracking, showing improved thermostability and performance comparable to existing systems.

This study identifies the mechanism of ATXN8OS CTA/CTG repeat-associated non-AUG translation through cell-free systems and live-cell imaging, revealing frameshifting and stress response involvement.

This paper presents an AI-driven method to convert antibody sequences into functional intrabodies, successfully converting 19 out of 26 sequences, including those targeting histone modifications.

The paper identifies four types of fragile nucleosomes that facilitate RNA Polymerase II transcription in human cells by analyzing histone modifications and Pol II activity.

PAbFold predicts linear antibody epitopes using AlphaFold2 by modeling antibody-antigen complexes, showing accuracy on known targets and validating on a novel anti-SARS-CoV-2 antibody.

The paper uses CRISPR-engineered cells to image MYH9 gene expression dynamics and shows translational buffering adjusts MYH9 protein levels in response to different cellular signals.

The paper shows that nonoptimal codon usage represses translation initiation, reducing protein levels independently of transcript abundance or stability.

This paper introduces GEARs, a toolkit for visualizing and manipulating endogenous proteins in vivo, demonstrating its use with Nanog and Vangl2 in zebrafish.

The paper uses live-cell imaging to show that H3K27ac and RNAP2-Ser5ph mark distinct chromatin sites with opposing nucleosome mobility dynamics.

This paper proposes a hybrid computational pipeline combining mechanistic models and machine learning to design single-color multiplexed Nascent Chain Tracking experiments capable of resolving multiple mRNA species.

The paper develops a method to visualize and quantify Ago2's impact on mRNA translation and localization in live cells, revealing dynamics of miRNA-mediated gene silencing.

The paper uses live-cell imaging to study RNA polymerase II phosphorylation dynamics at a single gene, revealing spatial and temporal organization of phosphorylation clusters during transcription.

The paper investigates how defects in the ubiquitin-proteasome system affect mRNA translation and partitioning into stress granules during acute stress.

The paper develops a computational model to simulate single-RNA translation dynamics and evaluates experimental assays for estimating elongation kinetics.

The paper images single mRNA interactions with stress granules and P-bodies during stress, showing dynamic and stable associations influenced by mRNA length and granule size.

This study identifies factors that determine Satb1 binding-site selectivity, showing its preference for nucleosome-dense regions and its mechanosensitive interactions with DNA.