Nature MethodsNeurosciencePeer-reviewed journal article
FACED 2.0 enables large-scale voltage and calcium imaging in vivo
Jian ZhongiD, Ryan Gregory NataniD, Qinrong ZhangiD, Justin S. J. WongiD, Christoph MiehliD, Krishnashish BoseiD, Xiaoyu LuiD, François St-PierreiD, Su GuoiD, Brent DoironiD, Kevin K. TsiaiD, Na JiiD
Abstract
Monitoring neuronal activity at large scale and high spatiotemporal resolution is crucial for understanding information processing within the brain. We optimized a kilohertz-frame-rate two-photon fluorescence microscope with all-optical megahertz line-scan rate to achieve ultrafast imaging across large areas and volumes at subcellular resolution. Applying this technique to voltage and calcium imaging in vivo, we demonstrated simultaneous recording of voltage activity over 200 neurons and calcium activity over 14,000 neurons.
- Citations
- 23
- FWCI
- 8.63
- Field percentile
- top 1%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- High-Speed Low-Light In Vivo Two-Photon Voltage Imaging of Large Neuronal Populations
Jelena Platiša, Xin Ye, … Jerry L. Chen·Nature MethodsPeer-reviewed·Mar 27, 2023
- Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
Jianglai Wu, Yajie Liang, … Na Ji·Nature MethodsPeer-reviewed·Mar 2, 2020
- Flexible Simultaneous Mesoscale Two-Photon Imaging of Neural Activity at High Speeds
Mitchell Clough, Anderson Chen, … Jerry L. Chen·Nature CommunicationsPeer-reviewed·Nov 17, 2021
- Fast scanning high optical invariant two-photon microscopy for monitoring a large neural network activity with cellular resolution
Keisuke Ota, Yasuhiro Oisi, … Masanori Murayama·bioRxiv·Jul 14, 2020
- Fast two-photon volumetric imaging of an improved voltage indicator reveals electrical activity in deeply located neurons in the awake brain
Vincent Villette, Mariya Chavarha, … Michael Z. Lin·CellPeer-reviewed·Dec 1, 2019
- Ultrafast Frame-Free Imaging of Neural Activity with Event Cameras
Angelo Forli, Krishna Chaitanya Kasuba, … Michael M. Yartsev·Current BiologyPeer-reviewed·Jul 1, 2026