Nature MethodsNeurosciencePeer-reviewed journal article
Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
Jianglai WuiD, Yajie LiangiD, Shuo CheniD, Ching-Lung HsuiD, Mariya ChavarhaiD, Stephen W. EvansiD, Dongqing ShiiD, Michael Z. LiniD, Kevin K. TsiaiD, Na JiiD
Abstract
Understanding information processing in the brain requires us to monitor neural activity in vivo at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope (2PFM) empowered by all-optical laser scanning, we imaged neural activity in vivo at up to 3,000 frames per second and submicron spatial resolution. This ultrafast imaging method enabled monitoring of both supra- and sub-threshold electrical activity down to 345 m below the brain surface in head fixed awake mice.
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