Single-cell analysis of signalling and transcriptional responses to type I interferons

Type I interferons (IFNs) play crucial roles in antiviral defence, autoinflammation and cancer immunity. The human genome encodes 17 different type I IFNs that all signal through the same receptor. Non-redundant functions have been reported for some type I IFNs. However, whether different type I IFNs induce different responses remains largely unknown. We stimulated human peripheral blood mononuclear cells (PBMCs) with recombinant type I IFNs to address this question in multiple types of primary cells. We analysed signalling responses by mass cytometry and changes in gene expression by bulk and single-cell RNA sequencing. We found cell-type specific changes in the phosphorylation of STAT transcription factors and in the gene sets induced and repressed upon type I IFN exposure. We further report that the magnitude of these responses varied between different type I IFNs, whilst qualitatively different responses to type I IFN subtypes were not apparent. Taken together, we provide a rich resource mapping signalling responses and IFN-regulated genes in immune cells. HighlightsO_LIMass cytometry and scRNAseq analysis of human PBMCs stimulated with type I IFNs C_LIO_LICell type-specific phosphorylation of STAT proteins and induction of ISGs C_LIO_LIDifferent type I IFNs induce qualitatively similar responses that vary in magnitude C_LIO_LIIdentification of ten core ISGs, up-regulated by all cell types in response to all type I IFNs C_LI In briefRigby et al. provide a single-cell map of signalling and transcriptomic responses to type I IFNs in ex vivo stimulated human PBMCs. Different cell types responded in unique ways but differences between different type I IFNs were only quantitative. These rich datasets are available via an easy-to-use interactive interface (https://rehwinkellab.shinyapps.io/ifnresource/). Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=195 HEIGHT=200 SRC="FIGDIR/small/547491v1_ufig1.gif" ALT="Figure 1"> View larger version (47K): org.highwire.dtl.DTLVardef@17e1e06org.highwire.dtl.DTLVardef@e53d81org.highwire.dtl.DTLVardef@290069org.highwire.dtl.DTLVardef@1da0c5a_HPS_FORMAT_FIGEXP M_FIG C_FIG