Longitudinal single cell fate in hematopoiesis in vivo using cellular barcoding and DiSNE movie visualization

Abstract

Identifying the progeny of many single progenitor cells simultaneously can be achieved by tagging progenitors with unique heritable DNA barcodes, and allows inferences of lineage relationships, including longitudinally. While this approach has shed new light on single cell fate heterogeneity, data interpretation remains a major challenge. In this study, we applied our developmental interpolated t-Distributed Stochastic Neighbor Embedding (DiSNE) movie approach to visualize the clonal dynamics of hematopoietic reconstitution in primates and identify novel developmental patterns, namely a potential cluster of hematopoietic progenitors with early T cell and later granulocyte production.\n\nKey pointsO_LIComplex single cell haematopoietic fate heterogeneity can be visualized and assessed with tSNE pie maps\nC_LIO_LIDiSNE movie visualization of in vivo haematopoiesis allows \"play back\" of the waves of haematopoiesis\nC_LIO_LIIdentification of novel hematopoietic progenitors with early T cell and later granulocyte production\nC_LI