Alejo Rodriguez-Fraticelli

A kinetics-based model of hematopoiesis reveals extrinsic regulation of skewed lineage output from stem cells

This study uses single-cell transplantation and mathematical modeling to show that lineage biases in hematopoiesis arise from extrinsic feedback and clonal competition, not intrinsic HSC properties.

Leukemia stem cell expansion cultures reveal clonal drivers of leukemogenesis and therapy response

This paper develops PLSTCs to expand AML LSCs and identifies clonal drivers of leukemogenesis and therapy response through genomic and single-cell analyses.

Somatic epimutations enable single-cell lineage tracing in native hematopoiesis across the murine and human lifespan

This paper introduces EPI-clone, a method using DNA methylation to trace clonal lineages in hematopoiesis without genetic engineering, showing clonal complexity declines with age in mice and expands in humans.

Pre-existing stem cell heterogeneity dictates clonal responses to acquisition of cancer driver mutations

The paper develops STRACK to trace clonal gene expression and expansion dynamics in leukemia, showing pre-existing stem cell heterogeneity dictates clonal responses to cancer mutations.

Reducing oxidative stress improves ex vivo polymer-based human haematopoietic stem and progenitor cell culture and gene editing

This study shows reducing oxidative stress enhances ex vivo growth of human haematopoietic stem and progenitor cells in polymer-based cultures and enables efficient gene editing.

A molecular switch: Ro5-3335 drives hematopoietic stem cell division and clonality through distinct RUNX1 binding partners

This study identifies Ro5-3335 as a chemical that increases HSC clone number and clonal diversity by altering RUNX1 binding partners in zebrafish and mouse models.

An engineered CRISPR/Cas9 mouse line for simultaneous readout of lineage histories and gene expression profiles in single cells

The paper introduces a CRISPR/Cas9 mouse line (CARLIN) to simultaneously track lineage histories and gene expression in single cells, revealing clonal bottlenecks in hematopoietic stem cells.

Lineage tracing on transcriptional landscapes links state to fate during differentiation

This study uses DNA barcodes to trace single-cell transcriptomes during differentiation, identifying fate biases and developmental hierarchies in hematopoiesis.

Single-cell time-series mapping of cell fate trajectories reveals an expanded developmental potential for human PSC-derived distal lung progenitors

The paper maps cell fate trajectories of human PSC-derived distal lung progenitors using scRNA-seq and identifies developmental plasticity regulated by Wnt signaling.