bioRxivBiophysicsPreprint
Reducing effects of particle adsorption to the air-water interface in cryoEM
Alex J. NobleiD, Hui Wei, Venkata P. DandeyiD, Zhening ZhangiD, Clinton S. PotteriD, Bridget CarragheriD
Abstract
Most protein particles prepared in vitreous ice for single particle cryo-electron microscopy are adsorbed to air-water or substrate-water interfaces, potentially causing particles to adopt preferred orientations. Using the Spotiton robot and nanowire grids, we can significantly reduce air-water interface issues by decreasing the dwell time of particles in thin liquid films. We demonstrate this by using single particle cryoEM and cryoET on three biological samples.
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