Gabriel C. Lander

This study uses cryo-EM to reveal structural plasticity and ligand promiscuity of CYP3A4, showing multiple conformations and highlighting the F/G loop's role in binding pocket remodeling.

This study compares the aggregation behavior of TTR variants from hummingbirds and elephants under mechanical agitation, revealing differences linked to their physiological adaptations.

This paper provides processing guidelines for helical reconstruction of amyloids using cryoSPARC, highlighting current capabilities and limitations while advocating for amyloid-specific parameter optimization.

This paper introduces DM, a detergent that reduces air-water interface artifacts in cryo-EM, improving reconstruction quality and particle stability.

This study shows that IXA4 inhibits SLC33A1, causing ER hyperoxidation and activating IRE1/XBP1s signaling, which reduces viability of KEAP1-deficient lung cancer cells.

This study uses proteomics and 3D imaging to show that ATTR cardiac amyloidosis involves microangiopathy, capillary occlusion, and vascular basement membrane congestion.

The paper investigates how the ultrastructure of biomolecular condensates, using PopZ as a model, influences their function through molecular interactions and phase-dependent conformational changes.

This study presents cryo-EM structures of human ClpXP, revealing mechanisms of assembly and proteolytic activation through conformational changes and interactions.

CryoSift automates 2D class selection in cryo-EM by using a CNN to assess particle quality, enabling high-throughput processing for high-resolution reconstructions.

The paper introduces Mix-it-up, a method for rapid on-grid mixing and vitrification in cryo-EM to capture fast biochemical processes, demonstrating its effectiveness with high-resolution structural analysis.

The paper reveals a cross-regulation between translation and NAD+ metabolism through the interaction of SerRS and SIRT2, mediated by ADP-ribose.

This study uses cryo-EM to identify a novel intermediate state of LONP1 that regulates substrate selection and activation, providing insights into its role in mitochondrial proteostasis.

Magellon provides a flexible and scalable cryo-EM platform with microservices architecture for data management, processing, and visualization.

This paper demonstrates that a 100 keV cryo-TEM with advanced components can achieve sub-3 Å resolution structures of various proteins and viruses.

This study uses cryo-EM to reveal the conformational landscape of human transthyretin, uncovering asymmetries and new states relevant to ligand binding and amyloid formation.

This study evaluates the Gatan Alpine detector at 100-200 keV for cryo-EM, showing improved resolution and performance compared to the Gatan K3.

This study redefines the hand-over-hand translocation mechanism of LONP1 using cryo-EM structures and biochemical assays, showing substrate binding regulates activity and ADP-bound ATPases can support translocation.

This paper reveals the molecular mechanism of polymerase theta helicase in positioning DNA microhomologies for error-prone double-strand break repair using cryo-electron microscopy structures.

This study reports the cryo-EM structure of DELE1's C-terminal fragment, showing its oligomerization and role in activating the integrated stress response.

This study investigates how CELMoD compounds induce conformational changes in cereblon, enabling neosubstrate binding and informing drug design strategies.

The paper determines the cryo-EM structure of yeast Lon protease (PIM1) and shows it forms a hexamer similar to bacterial and human Lon proteases, with unique structural features and a dispensable yeast-specific insertion.

This paper presents cryo-EM structures of Piwi-piRNA complexes and analyzes their target recognition mechanism, revealing how Piwi ensures targeting fidelity and adapts to genomic threats.

This study determines the cryo-EM structures of human LONP1 to elucidate the molecular mechanism of its proteolytic activation and regulation.

The paper introduces a user-guided refinement approach for subtomogram averaging to improve alignment of structurally complex macromolecular assemblies.

This study determines the structure of AFG3L2, revealing how its unique features influence enzymatic activity and disease-related mutations.

The paper determines the cryo-EM structure of Y. pestis Lon protease during substrate processing, revealing a distinct hand-over-hand translocation mechanism unique to the HCLR clade.

The paper presents the cryo-EM structure of the type I-F CRISPR surveillance complex and shows a conformational change that exposes a nuclease recruitment helix homologous to an anti-CRISPR protein.

The paper presents cryo-EM structures of the 26S proteasome in four conformational states to elucidate how ATP hydrolysis drives substrate translocation and deubiquitination.

The paper explores using crowdsourcing for cryo-EM particle picking, showing its potential and limitations through experiments with untrained citizen scientists.

The paper presents the first atomic structure of the mitochondrial inner membrane AAA+ protease YME1, revealing its mechanism of substrate processing and translocation.