Frontiers in Cellular NeuroscienceNeurosciencePeer-reviewed journal article
Analysis of microglial BDNF function and expression in the motor cortex
Diana Honey, Erin WosnitzkaiD, Eric KlanniD, Laetitia WeinhardiD
Abstract
Brain-derived neurotrophic factor (BDNF) is a neurotrophin that regulates several aspects of brain function. Although numerous studies have demonstrated the expression and function of BDNF in neurons, its expression in microglia remains controversial. Using a combination of genetic tools and fluorescence imaging, we analyzed BDNF expression pattern and interrogated the effect of microglial BDNF deletion on neuronal activity, early-stage spine formation, and microglia-neuron interactions in the motor cortex. Our results suggest that microglia do not express BDNF in sufficient amounts to modulate neuronal function.
- organism: mouse
- research focus: cell-cell communication
- research focus: differential expression
- research focus: gene regulatory network inference
- approach: experimental assay
- Citations
- 13
- FWCI
- 1.14
- Field percentile
- top 23%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Genetic dissection of BDNF and TrkB expression in glial cells
Changran Niu, Xinpei Yue, … Baoji Xu·BiomoleculesPeer-reviewed·Jan 11, 2024
- Neuronal activity patterns regulate BDNF expression in cortical neurons via synaptic connections and calcium signaling
Yumi Miyasaka, Nobuhiko Yamamoto·bioRxiv·Feb 28, 2021
- A novel enhancer that regulates Bdnf expression in developing neurons
Emily Brookes, Ho Yu Alan Au, … Antonella Riccio·bioRxiv·Nov 18, 2021
- Microglial brain-derived neurotrophic factor (BDNF) supports the behavioral and synaptogenic effects of ketamine
Samuel C. Woodburn, Alexander M. Kuhn, … Eric S. Wohleb·Brain Behavior and ImmunityPeer-reviewed·Jul 2, 2026
- Visualizing BDNF cell-to-cell transfer reveals astrocytes are the primary recipient of neuronal BDNF
Markus A. Stahlberg, Sebastian Kügler, Camin Dean·bioRxiv·Mar 8, 2018
- Neuronal network activity controls microglial process surveillance in awake mice via norepinephrine signaling
Yong Liu, Yanlu Ying, … Long‐Jun Wu·Nature NeurosciencePeer-reviewed·Oct 21, 2019