Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution

Hao Wang; Qingyuan Zhu; Lufeng Ding; Yan Xia Shen; Chao‐Yu Yang; Fang Xu; Chang Shu; Yujie Guo; Zhiwei Xiong; Qing‐Hong Shan; Fan Jia; Peng Su; Qianru Yang; Bing Li; Yuxiao Cheng; Xiaobin He; Xi Chen; Feng Wu; Jiang‐Ning Zhou; Fuqiang Xu; Hua Han; Pak-Ming Lau; Guo‐Qiang Bi

doi:10.1093/nsr/nwz053

NeuroscienceNational Science ReviewApr 15, 2019

Peer-reviewed journal article

Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution

Hao Wang iD, Qingyuan Zhu iD, Lufeng Ding, Yan Xia Shen, Chao‐Yu Yang iD, Fang Xu iD, Chang Shu iD, Yujie Guo iD, Zhiwei Xiong iD, Qing‐Hong Shan iD, Fan Jia iD, Peng Su iD, Qianru Yang iD, Bing Li iD, Yuxiao Cheng iD, Xiaobin He iD, Xi Chen iD, Feng Wu iD, Jiang‐Ning Zhou iD, Fuqiang Xu iD, Hua Han iD, Pak-Ming Lau iD, Guo‐Qiang Bi iD

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Abstract

We describe a new light-sheet microscopy method for fast, large-scale volumetric imaging. Combining synchronized scanning illumination and oblique imaging over cleared, thick tissue sections in smooth motion, our approach achieves high-speed 3D image acquisition of an entire mouse brain within 2 hours, at a resolution capable of resolving synaptic spines. It is compatible with immunofluorescence labeling, enabling flexible cell-type specific brain mapping, and is readily scalable for large biological samples such as primate brain.