HaloTag9: an engineered protein tag to improve fluorophore performance
M. S. Frei, M. Tarnawski, J. Roberti, B. Koch, J. Hiblot, K. Johnsson
Abstract
HaloTag9 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag9 with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased brightness of HaloTag9 and its use in fluorescence lifetime multiplexing makes it a powerful tool for live-cell imaging.
02 /Specification & Classification
Click any tag to browse matching papers- Assay
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Nanobodies equipped with HaloTag variants enable rapid and straightforward one-step immunofluorescence lifetime multiplexing
László Albert, Samrat Basak, … Felipe Opazo·bioRxiv·Mar 2, 2026
- Exchangeable HaloTag Ligands (xHTLs) for multi-modal super-resolution fluorescence microscopy
J. Kompa, J. Bruins, … K. Johnsson·bioRxiv·Jun 20, 2022
- Multiplexed in vivo imaging with fluorescence lifetime modulating tags
Lina El Hajji, France Lam, … Arnaud Gautier·Advanced SciencePeer-reviewed·Jun 20, 2024
- A photoswitchable HaloTag for spatiotemporal control of fluorescence in living cells
Franziska Walterspiel, Begoña Ugarte‐Uribe, … Claire Deo·Angewandte Chemie International EditionPeer-reviewed·Oct 23, 2025
- A high-affinity split-HaloTag for live-cell protein labeling
Yin‐Hsi Lin, Julian Kompa, … Kai Johnsson·Nature CommunicationsPeer-reviewed·Mar 25, 2026
- Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging
Julian Kompa, L Dornfeld, … Kai Johnsson·bioRxiv·Jul 6, 2025