Author

M. S. Frei

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 3 Bioengineering· 1 Biophysics· 1 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Assay:fluorescence / live-cell microscopy· 5 Research Focus:cell type annotation· 3 Research Focus:imputation· 3 Research Focus:cell signaling & trafficking· 2 Assay:imaging· 2 Approach:microscopy & imaging analysis· 1 Organism:mouse· 1

Frequent Co-Authors

K. Johnsson· 5 shared J. Hiblot· 4 shared B. Koch· 3 shared M. Tarnawski· 3 shared A. Bergner· 1 shared A. C. Lyons· 1 shared A. Edenhofer· 1 shared B. Nijmeijer· 1 shared

Activity by Year

2019-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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5 shared papersBiochemistry

K. Johnsson

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging

M. S. Frei, S. A. Sanchez, L. Liu, F. Schneider, Z. Wang, H. Hakozaki, Y. Li, A. C. Lyons, T. V. Rohm, J. M. Olefsky, L. Shi, J. Schöneberg, S. E. Fraser, S. Mehta, Y. Wang, J. Zhang

The paper introduces far-red chemigenetic kinase activity biosensors enabling five-color multiplexing, 4D imaging, and super-resolution via STED microscopy.

bioRxiv · Feb 10, 2024 · PreprintBioengineering

A general method for the development of multicolor biosensors with large dynamic ranges

L. Hellweg, A. Edenhofer, L. Barck, M.-C. Huppertz, M. S. Frei, M. Tarnawski, A. Bergner, B. Koch, K. Johnsson, J. Hiblot

The paper introduces a method to create multicolor biosensors with large dynamic ranges using FRET pairs based on fluorescent proteins and HaloTag.

bioRxiv · Nov 29, 2022Biochemistry

Exchangeable HaloTag Ligands (xHTLs) for multi-modal super-resolution fluorescence microscopy

J. Kompa, J. Bruins, M. Glogger, J. Wilhelm, M. S. Frei, M. Tarnawski, E. d'Este, M. Heilemann, J. Hiblot, K. Johnsson

The paper introduces exchangeable HaloTag ligands for multi-modal super-resolution fluorescence microscopy, enabling dual-color imaging techniques like PAINT and STED.

bioRxiv · Jun 20, 2022 · PreprintBiochemistry

Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes

M. S. Frei, B. Koch, J. Hiblot, K. Johnsson

The paper demonstrates that eight pairwise combinations of 18 synthetic fluorescent probes are suitable for fluorescence lifetime multiplexing in mammalian cells, enabling imaging of up to six biological targets.

bioRxiv · Jan 15, 2022Cell Biology

HaloTag9: an engineered protein tag to improve fluorophore performance

M. S. Frei, M. Tarnawski, J. Roberti, B. Koch, J. Hiblot, K. Johnsson

HaloTag9 improves fluorophore performance with higher brightness and fluorescence lifetime, enabling live-cell multiplexing and biosensor generation.

Nature Methods · Dec 16, 2021Peer-reviewedBiochemistry

Photoactivation of silicon rhodamines via a light-induced protonation

M. S. Frei, P. Hoess, M. Lampe, B. Nijmeijer, M. Kueblbeck, J. Ellenberg, J. Ries, S. Pitsch, L. Reymond, K. Johnsson

This paper introduces a new photoactivatable fluorophore that enables live-cell super-resolution microscopy without caging groups or side-products.

bioRxiv · May 4, 2019Biophysics