Clinical ChemistryMolecular BiologyPeer-reviewed journal article
Leukocyte counts based on site-specific DNA methylation analysis
Joana Frobel, Tanja Božić, Michael Lenz, Peter Uciechowski, Yang HaniD, Reinhild Herwartz, Klaus Strathmann, Susanne IsfortiD, Jens PanseiD, André EsseriD, Carina Birkhofer, Uwe Gerstenmaier, Thomas KrausiD, Lothar RinkiD, Steffen KoschmiederiD, Wolfgang WagneriD
Abstract
The composition of white blood cells is usually assessed by histomorphological parameters or flow cytometric measurements. Alternatively, leukocyte differential counts (LDCs) can be estimated by deconvolution algorithms for genome-wide DNA methylation (DNAm) profiles. We identified cell-type specific CG dinucleotides (CpGs) that facilitate relative quantification of leukocyte subsets. Site-specific analysis of DNAm levels by pyrosequencing provides similar precision of LDCs as conventional methods, whereas it is also applicable to frozen samples and requires only very small volumes of blood. Furthermore, we describe a new approach for absolute quantification of cell numbers based on a non-methylated reference DNA. Our \"Epi-Blood-Count\" facilitates robust and cost effective analysis of blood counts for clinical application.
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