Naphthalimide-based, Single-Chromophore, Ratiometric Fluorescent Sensor for Tracking Intracellular pH

Sujit Kumar Das; Smitaroopa Kahali; Sabnam Kar; Nandita Madhavan; Ankona Datta

doi:10.1002/cbic.202400538

BiochemistryChemBioChemJul 29, 2024

Peer-reviewed journal article

Naphthalimide-based, Single-Chromophore, Ratiometric Fluorescent Sensor for Tracking Intracellular pH

Sujit Kumar Das iD, Smitaroopa Kahali iD, Sabnam Kar iD, Nandita Madhavan iD, Ankona Datta iD

Abstract

We report a novel, reversible, cell-permeable, pH-sensor, TRapH. TRapH afforded a pH-sensitive ratiometric emission response in the pH range [~]3-6, enabling imaging and quantification of pH in living cells. The biological-applicability of TRapH was illustrated via live-tracking of intracellular pH dynamics in living mammalian cells induced by a synthetic H+-transporter.

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