Molecular Sensors and Ion Detection

Leveraging Chlorination-Based Mechanism for Resolving Subcellular Hypochlorous Acid

This paper introduces HOClSense dyes, a chlorination-based indicator for subcellular HOCl detection, and demonstrates their use in identifying STING pathway-induced HOCl and abnormal HOCl production in Niemann-Pick diseases.

Coumarin-based blue excitable probe for functional screening of dehalogenase activity

This paper introduces a new fluorescent probe, COU4-Cl, excitable at 405 nm and 488 nm, for detecting dehalogenase activity, and compares it to the existing COU2-Cl probe.

Rhodamine-derived ratiometric fluorescent molecular rotor for mitochondrial viscosity sensing

This paper introduces a new ratiometric fluorescent molecular rotor for mitochondrial viscosity sensing, showing it can quantify inner mitochondrial membrane viscosity and reveal heterogeneity under oxidative stress.

Evaluation of selectively-activatable, caged fluorescent probes as species selective markers for beta-alanine aminopeptidase positive bacterial species

The paper evaluates caged fluorescent probes for detecting beta-alanine aminopeptidase activity in bacterial species, showing selectivity for Pseudomonas aeruginosa, Burkholderia, and some Serratia strains, while being inactive in others.

Near-Infrared Turn-On Fluorogenic Probe for Versatile Detection of Inorganic Polyphosphates

This paper introduces a near-infrared fluorogenic probe for selective detection and imaging of inorganic polyphosphates in various biological contexts.

Rosindol: A fluorogen for the quantitative measurement of reactive oxygen species in living cells

Rosindol is a new fluorogenic probe that accurately measures reactive oxygen species in living cells with linear responses and pH independence.

Single-Molecule Activity Profiling of Glycosidase Proteoforms Using Water-Soluble Fluorogenic Probes

This study develops water-soluble fluorogenic probes for single-molecule glycosidase activity profiling in blood, revealing -mannosidase as a potential biomarker for liver injury.

Localizable fluorescent metal ion indicators with tunable colors

The paper introduces a modular strategy to create fluorescent metal ion indicators with tunable colors and subcellular localization, demonstrating their use in live-cell potassium sensing in rat hippocampal neurons.

Low-Background Cancer Imaging With a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety

The paper develops a bioorthogonal fluorescence probe and engineered enzyme for low-background cancer imaging in mouse models.

A ratiometric pH sensor for Gram-positive and Gram-negative bacteria

The paper develops a ratiometric fluorescent pH sensor for Gram-positive and Gram-negative bacteria, validated in E. coli, S. epidermidis, and MRSA, enabling real-time pH tracking and identification of pH-sensitive phenotypes.

Targeted fluorescent lipid microparticles for quantitative measurement of phagosomal pH

This paper develops fluorescent lipid microparticles that measure phagosomal pH in real-time by combining pH-responsive dyes with a reference probe.

Cycling Molecular Assemblies (CyMA) for Ultrasensitive Golgi Imaging

The paper introduces BODIPY-CyMA, a probe that uses enzymatic cycles for ultrasensitive Golgi imaging with low concentrations and minimal incubation.

Visualizing the Chronicle of Multiple Cell Fate using a Near-IR Dual-RNA/DNA-Targeting Probe

This paper introduces a near-IR dual-RNA/DNA-targeting probe that enables discrimination of multiple cell states by detecting RNA-DNA changes, offering greater informational content than existing fluorophores.

Prodan-based solvatochromic probes for polarity imaging of organelles

The paper introduces Prodan-based solvatochromic probes for imaging organelle polarity, showing organelle-specific polarity changes under starvation.

Strategic Modulation of Polarity and Viscosity Sensitivity of Bimane Molecular Rotor-Based Fluorophores for Imaging α-Synuclein

This paper designs new bimane-based fluorophores sensitive to polarity and viscosity for imaging α-synuclein in Parkinson's disease.

A Near Infrared Fluorescent Nanosensor for Spatial and Dynamic Measurements of Auxin, Indole-3-Acetic Acid, in Planta

This paper introduces a near infrared fluorescent nanosensor for spatial and dynamic measurement of IAA in planta, showing species independence and functionality in plant cells.

Fluorescent anionic cyanine plasma membrane probes for live cell and in vivo imaging

The paper introduces anionic cyanine-based plasma membrane probes for live cell and in vivo imaging, showing improved staining and visualization of neuronal structures in mice.

RNA Mango-based Sensors for Lead

This paper develops RNA Mango-based sensors for detecting lead, showing high sensitivity and selectivity with low detection limits.

Cellularly-Retained Fluorogenic Probes for Sensitive Cell-Resolved Bioactivity Imaging

This paper develops a fluorogenic probe scaffold that retains fluorescent products within cells to improve imaging sensitivity and resolution for quantifying low-turnover biological processes.

Modular Design and Scaffold-Synthesis of Multi-Functional Fluorophores for Targeted Cellular Imaging and Pyroptosis

This study designs and synthesizes functional fluorophores using a scaffold-based Ugi reaction, enabling detection of reactive-oxygen species and controlled subcellular localization, with some compounds showing pyroptosis-inducing activity in cancer cells.

Tunable Cytosolic Chloride Indicators for Real-Time Chloride Imaging in Live Cells

This paper develops CytoCl dyes for real-time imaging of cytosolic chloride flux in live cells, revealing rapid changes and enabling first-time single-cell imaging of chloride channel activity.

Imaging membrane damage in ferroptosis and necrosis by wash-free fluorogenic chemical probes

The paper develops fluorogenic chemical probes that detect membrane damage without washing steps, enabling in vivo imaging of cell death mechanisms like ferroptosis and necrosis.

A Novel Fluorogenic Spin Probe for Real-time Monitoring of Mitochondrial Reactive Oxygen Species (mROS) Generation in an in vitro Stroke Model

Fluorescent and bioluminescent calcium indicators with tuneable colors and affinities

This paper introduces a family of tunable calcium indicators with fluorescence and bioluminescence readouts, applicable for live-cell imaging and high-throughput assays in rat neurons.

High Throughput Evolution of Near Infrared Serotonin Nanosensors

This paper develops a near-infrared serotonin nanosensor through synthetic evolution, showing high selectivity and affinity for serotonin over other compounds and enabling reversible imaging in brain slices.

A chemogenetic approach for optical monitoring of voltage in neurons

The paper introduces a chemogenetic tool for optical voltage monitoring in neurons using a fluorescent probe that detects both supra- and subthreshold activity.