Bioscience Biotechnology and BiochemistryGeneticsPeer-reviewed journal article
Generation of Temperature Sensitive Mutations with Error-Prone PCR in a Gene Encoding a Component of the Spindle Pole Body in Fission Yeast
Ngang Heok TangiD, Chii Shyang FongiD, Hirohisa Masuda, Isabelle JourdainiD, Masashi YukawaiD, Takashi TodaiD
Abstract
Temperature-sensitive (ts) mutants provide powerful tools, thereby investigating cellular functions of essential genes. We report here a simple procedure to generate ts mutations using error-prone PCR in pcp1 that encodes a spindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to analysis of Pcp1, and can be suited to any essential genes involved in other processes.
- Citations
- 11
- FWCI
- 0.33
- Field percentile
- top 48%
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