Raphaël Loll‐Krippleber

pSPObooster: a plasmid system to improve sporulation efficiency of Saccharomyces cerevisiae lab strains

The paper introduces pSPObooster, a plasmid system that improves sporulation efficiency in S. cerevisiae lab strains by correcting meiotic defects in MKT1 and RME1 genes.

Logan: Planetary-Scale Genome Assembly Surveys Life's Diversity

Assembling the Sequence Read Archive (27.3 million accessions) with over 100-fold compression enables minutes-scale k-mer search and mining of enzymes, plasmids, proteins and viral reactivation.

A conserved function of corepressors is to nucleate assembly of the transcriptional preinitiation complex.

The paper identifies TPL's role in nucleating the transcriptional preinitiation complex in yeast and plants through genetic and physical interaction studies.

Mec1-Independent Activation of the Rad53 Checkpoint Kinase Revealed by Quantitative Analysis of Protein Localization Dynamics

The paper identifies Rad53-dependent protein localization changes during replication stress, revealing Mec1-independent activation of Rad53 in yeast.

Development of a yeast whole-cell biocatalyst for MHET conversion into terephthalic acid and ethylene glycol

This study develops a yeast whole-cell biocatalyst expressing MHETase for PET degradation, showing comparable activity to purified enzyme and stability under various conditions.

Distinct ribosome states trigger diverse mRNA quality control pathways

The paper investigates mRNA quality control pathways in yeast, showing that Syh1 is central to no-go decay (NGD) while Not5 has a minor role, and ribosome profiling reveals distinct ribosomal states for NGD and COMD.

Complex Mutation Profiles in Mismatch Repair and Ribonucleotide Reductase Mutations Reveal Novel Repair Substrate Specificity of MutS Homolog (MSH) Complexes

The paper identifies mutation signatures associated with MMR pathway defects by combining rnr1 and msh mutations, revealing novel repair substrate specificities of Msh2-Msh3 and Msh2-Msh6 complexes.

Phenotypic Heterogeneity in the DNA Replication Stress Response Revealed by Quantitative Protein Dynamics Measurements

The paper quantifies protein localization dynamics in yeast during DNA replication stress, revealing phenotypic heterogeneity and previously undetected re-localization events.

Genetic background and mistranslation frequency determine the impact of mistranslating tRNASerUGG

This study shows that the impact of a mistranslating tRNA variant depends on genetic background and mistranslation frequency in yeast, with implications for understanding tRNA variants in human disease.

Genetic screens identify connections between ribosome recycling and nonsense mediated decay

The paper identifies factors linking ribosome recycling and nonsense mediated decay in yeast using genetic screens and reporter constructs.

The amino acid substitution affects cellular response to mistranslation

This study compares the effects of two tRNA variants causing different amino acid substitutions in yeast, showing distinct impacts on growth, heat shock response, and transcriptome changes.

Chemical-genetic interactions with the proline analog L-azetidine-2-carboxylic acid in Saccharomyces cerevisiae

The paper identifies genes with chemical-genetic interactions with AZC in yeast, revealing pathways involved in coping with amino acid mis-incorporation and proteotoxic stress.

Mistranslating tRNA identifies a deleterious S213P mutation in the Saccharomyces cerevisiae eco1-1 allele

The paper identifies a deleterious S213P mutation in the eco1-1 allele using mistranslating tRNA variants that suppress its temperature sensitivity.

The endonuclease Cue2 cleaves mRNAs at stalled ribosomes during No Go Decay.

The paper identifies Cue2 as the endonuclease responsible for cleaving mRNAs at stalled ribosomes during No Go Decay and shows it acts as a secondary decay pathway.