Grant W. Brown

PRMT1 arginine methylation of MCM4 restricts ssDNA gap formation during DNA replication

This study identifies PRMT1 as a replisome-associated methyltransferase that suppresses ssDNA gap formation by methylating MCM4, thereby maintaining fork integrity during DNA replication.

A global genetic interaction map of a human cell reveals conserved principles of genetic networks

The paper constructs a genome-scale genetic interaction network from 4 million double mutants in HAP1 cells, revealing conserved genetic network principles across species and uncovering novel gene functions and cancer dependencies.

The BLM-TOP3A-RMI1-RMI2 proximity map reveals that RAD54L2 suppresses sister chromatid exchanges

The paper identifies RAD54L2 as a regulator of recombination by showing its interaction with the BTRR complex and its role in suppressing sister chromatid exchanges.

pSPObooster: a plasmid system to improve sporulation efficiency of Saccharomyces cerevisiae lab strains

The paper introduces pSPObooster, a plasmid system that improves sporulation efficiency in S. cerevisiae lab strains by correcting meiotic defects in MKT1 and RME1 genes.

Logan: Planetary-Scale Genome Assembly Surveys Life's Diversity

Assembling the Sequence Read Archive (27.3 million accessions) with over 100-fold compression enables minutes-scale k-mer search and mining of enzymes, plasmids, proteins and viral reactivation.

The cytidine deaminase APOBEC3C has unique sequence and genome feature preferences

This study defines the mutation signature and genome feature preferences of APOBEC3C, showing it differs from APOBEC3A and APOBEC3B in mutation clustering and strand bias.

A conserved function of corepressors is to nucleate assembly of the transcriptional preinitiation complex.

The paper identifies TPL's role in nucleating the transcriptional preinitiation complex in yeast and plants through genetic and physical interaction studies.

Two independent DNA repair pathways cause mutagenesis in template switching deficient Saccharomyces cerevisiae

The paper investigates two DNA repair pathways in yeast and shows they contribute to mutagenesis when error-free repair is compromised.

Mec1-Independent Activation of the Rad53 Checkpoint Kinase Revealed by Quantitative Analysis of Protein Localization Dynamics

The paper identifies Rad53-dependent protein localization changes during replication stress, revealing Mec1-independent activation of Rad53 in yeast.

Development of a yeast whole-cell biocatalyst for MHET conversion into terephthalic acid and ethylene glycol

This study develops a yeast whole-cell biocatalyst expressing MHETase for PET degradation, showing comparable activity to purified enzyme and stability under various conditions.

Distinct ribosome states trigger diverse mRNA quality control pathways

The paper investigates mRNA quality control pathways in yeast, showing that Syh1 is central to no-go decay (NGD) while Not5 has a minor role, and ribosome profiling reveals distinct ribosomal states for NGD and COMD.

Complex Mutation Profiles in Mismatch Repair and Ribonucleotide Reductase Mutations Reveal Novel Repair Substrate Specificity of MutS Homolog (MSH) Complexes

The paper identifies mutation signatures associated with MMR pathway defects by combining rnr1 and msh mutations, revealing novel repair substrate specificities of Msh2-Msh3 and Msh2-Msh6 complexes.

Phenotypic Heterogeneity in the DNA Replication Stress Response Revealed by Quantitative Protein Dynamics Measurements

The paper quantifies protein localization dynamics in yeast during DNA replication stress, revealing phenotypic heterogeneity and previously undetected re-localization events.

Genetic background and mistranslation frequency determine the impact of mistranslating tRNASerUGG

This study shows that the impact of a mistranslating tRNA variant depends on genetic background and mistranslation frequency in yeast, with implications for understanding tRNA variants in human disease.

Genetic screens identify connections between ribosome recycling and nonsense mediated decay

The paper identifies factors linking ribosome recycling and nonsense mediated decay in yeast using genetic screens and reporter constructs.

Topoisomerase II deficiency leads to a postreplicative structural shift in all Saccharomyces cerevisiae chromosomes.

This study shows that Top2 deficiency in yeast causes a structural shift in chromosomes after replication, leading to segregation issues without triggering G2/M checkpoints.

The amino acid substitution affects cellular response to mistranslation

This study compares the effects of two tRNA variants causing different amino acid substitutions in yeast, showing distinct impacts on growth, heat shock response, and transcriptome changes.

Chemical-genetic interactions with the proline analog L-azetidine-2-carboxylic acid in Saccharomyces cerevisiae

The paper identifies genes with chemical-genetic interactions with AZC in yeast, revealing pathways involved in coping with amino acid mis-incorporation and proteotoxic stress.

Mistranslating tRNA identifies a deleterious S213P mutation in the Saccharomyces cerevisiae eco1-1 allele

The paper identifies a deleterious S213P mutation in the eco1-1 allele using mistranslating tRNA variants that suppress its temperature sensitivity.

A genome-wide screen for genes affecting spontaneous direct-repeat recombination in Saccharomyces cerevisiae

The paper identifies 35 genes that, when deleted, cause hyper-recombination between direct repeats in yeast, including mismatch repair genes and DNA replication factors.

The endonuclease Cue2 cleaves mRNAs at stalled ribosomes during No Go Decay.

The paper identifies Cue2 as the endonuclease responsible for cleaving mRNAs at stalled ribosomes during No Go Decay and shows it acts as a secondary decay pathway.

Mapping DNA damage-dependent genetic interactions in yeast via party mating and barcode fusion genetics

The paper introduces BFG-GI to map DNA damage-dependent genetic interactions in yeast by combining party mating and barcode fusion genetics, revealing new condition-specific interactions.

Comparative analysis of protein abundance studies to quantify the Saccharomyces cerevisiae proteome

This study normalizes and compares 19 yeast proteomic datasets to estimate absolute protein abundance and assess measurement variability across stress conditions.

Genome-scale genetic interactions position the Mitotic Exit Network as a major antagonist of transient Topoisomerase II deficiency.

The paper identifies the Mitotic Exit Network as a protective factor against Top2 deficiency by comparing yeast strains with different Top2 alleles.

Functional Annotation of Chemical Libraries across Diverse Biological Processes

This study develops a yeast-based chemical-genetic screening system to annotate compound functionality across biological processes and identifies compounds with dual modes of action.