Qualitative assessment of cryo-EM map reconstruction

Abstract

With the rapid advancement of Cryo-EM technique in deciphering structural features of biological molecules, it is a necessity to develop and explore different parameters which help us in critical evaluation of different aspects of reconstruction. In this age of ever-increasing automation and number of particles used in reconstruction to achieve high resolution maps, assessment parameters help in validation of reconstruction process. Here, we have employed one such quality parameter which quantitatively assess the presence of non-particles in the reconstructed map.