M. A. Herzik

Structural insights into GrpEL1-mediated nucleotide and substrate release of human mitochondrial Hsp70

The paper determines the structure of human mortalin-GrpEL1 complexes using cryoEM and identifies mechanisms of nucleotide and substrate release in the Hsp70 system.

Tuning ice thickness using the chameleon for high-quality cryoEM data collection

This paper describes the use of the chameleon device for tuning ice thickness to improve cryoEM grid quality and image quality.

CryoEM Structures of the Nitrogenase Complex During Catalytic Turnover

High-resolution cryoEM structures of the nitrogenase complex obtained under turnover provide new mechanistic insights.

Outcomes of the 2019 EMDataResource model challenge: validation of cryo-EM models at near-atomic resolution

The paper evaluates cryo-EM model quality using benchmark maps, showing high accuracy and reproducibility across 13 teams and recommending multiple metrics for model assessment.

Sub-2 A Resolution Structure Determination Using Single-Particle Cryo-EM at 200 keV

This study shows that a 200 keV cryo-EM microscope can achieve 1.7 Å resolution for macromolecular structures, comparable to higher voltage systems.

High-resolution structure determination of sub-100 kilodalton complexes using conventional cryo-EM

The paper demonstrates that conventional cryo-EM can determine high-resolution structures of sub-100 kDa complexes, resolving ligands and conformational states with sub-nanometer precision.

Conformational ensemble of the human TRPV3 ion channel

The paper presents cryo-EM structures of human TRPV3 in different states to elucidate its sensitization and gating mechanisms.

Achieving Better Than 3 A Resolution By Single Particle Cryo-EM At 200 keV

This paper shows that 200 keV TEMs can achieve cryo-EM resolutions better than 3 Å, enabling detailed structural analysis previously thought possible only with 300 keV TEMs.

A multi-model approach to assessing local and global cryo-EM map quality

The paper presents a multi-model approach to assess local and global cryo-EM map quality using independent model generation and convergence analysis.