Nature MethodsNeurosciencePeer-reviewed journal article
Quantitative mRNA Imaging Throughout the Entire Drosophila Brain
Xi LongiD, Jennifer I. ColonelliD, Allan M. WongiD, Robert H. SingeriD, Timothée LionnetiD
Abstract
We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid, multiplexable and does not require molecular amplification; it allows facile mRNA expression quantification with subcellular resolution on a standard confocal microscope. Using a custom Bessel Beam-Structured Illumination microscope (BB-SIM), we further demonstrate single-mRNA detection across the entire brain sample.
- organism: Drosophila
- assay: spatial transcriptomics
- research focus: cell type annotation
- approach: experimental assay
- Citations
- 91
- FWCI
- 8.02
- Field percentile
- top 1%
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