Robert H. Singer

This study uses live-cell imaging to show that nonsense-mediated mRNA decay triggers a transcriptional feedback loop.

The paper uses integrated imaging and omics approaches to show that Actb and Arc mRNAs are packaged into distinct granules with different trafficking dynamics and functional roles.

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

The paper shows that CLB2 mRNA localization to the bud via the She2/She3 complex enhances translation, linking cell growth to cell cycle progression through a ZIP-code element in the coding sequence.

The paper shows that mRNAs condense broadly in yeast under stress, independent of length, and new transcripts escape this condensation for selective translation.

The paper uses single-molecule imaging to show activity-dependent localization and translation of Camk2a mRNA at dendritic spines, revealing a mechanism for maintaining CaMKII levels during synaptic plasticity.

The paper identifies abundant RNA-binding proteins in mouse lenses and characterizes their interactions with crystallin and β-actin mRNAs using proteomic, transcriptomic, and imaging techniques.

This study shows that phosphorylation of FMRP at S499 alters granule structure and function, affecting transport and translation in neurons.

This study uses real-time imaging to show that Arc/Arg3.1 transcription in neurons varies based on the type of synaptic input, revealing distinct dynamics for medial and lateral perforant path activation.

This study uses single-molecule imaging to visualize miRNA-mediated gene silencing in cells, revealing miRNAs bind to translated mRNAs and induce repression and decay within 30-60 minutes.

The paper investigates how transcription and translation cycles of the IEG Arc support long-term memory in the hippocampus by maintaining mRNA supply to dendrites.

The paper identifies DNA base-excision repair as a mechanism that amplifies transcriptional noise, enhancing cellular plasticity and reprogramming efficiency.

This study shows that presynaptic FMRP and local protein synthesis regulate structural and functional plasticity in hippocampal mossy fiber-CA3 synapses.

The paper creates a comprehensive atlas of the Drosophila larval nervous system using single-cell transcriptomics and validates findings with RNA-FISH, revealing gene expression changes linked to behavioral states.

The paper introduces a smFISH method for single-mRNA detection in cleared Drosophila brains with subcellular resolution using a custom microscope.