Stem Cell ReportsDevelopmental BiologyPeer-reviewed journal article
MIXL1 Activation in Endoderm Differentiation ofHuman Induced Pluripotent Stem Cells
Pierre OsteiliD, Sarah WitheyiD, Nicole Santucci, Nader AryamaneshiD, Ignatius Pang, Nazmus SalehiniD, Jane Q. J. Sun, Annie Qin, Jiayi SuiD, Hilary KnowlesiD, Xiucheng Bella Li, Simon Cai, Ernst Jurgen WolvetangiD, Patrick TamiD
Abstract
Human induced pluripotent stem cells (hiPSC) possess the ability to differentiate into a multitude of cell and tissue types but display heterogeneous propensity of differentiation into specific lineage. Characterization of the transcriptome of eleven hiPSC lines showed that activation of MIXL1 at the early stage of stem cell differentiation correlated with higher efficacy in generating definitive endoderm and advancing differentiation and maturation of endoderm derivatives. Enforced expression of MIXL1 in the endoderm-inefficient hiPSCs enhanced the propensity of endoderm differentiation, suggesting that modulation of key drivers of lineage differentiation can re-wire hiPSC to the desired lineage propensity to generate the requisite stem cell products.
- organism: human
- assay: bulk RNA-seq
- research focus: differential expression
- approach: statistical model
- Citations
- 3
- FWCI
- 0.90
- Field percentile
- top 32%
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Endogenous suppression of WNT signalling in human embryonic stem cells leads to low differentiation propensity towards definitive endoderm
D. Dziedzicka, M. Tewary, … M. Geens·bioRxiv·Jul 3, 2020
- Developmental regulation of endothelial-to-hematopoietic transition from induced pluripotent stem cells
Rachel Wellington, Xiaoyi Cheng, … Sergei R. Doulatov·Stem Cell ReportsPeer-reviewed·Sep 23, 2025
- The transcription factor FoxO1 is required for the establishment of the human definitive endoderm
Joshua A. Nord, Daniel Schill, … Lisa Ann Cirillo·bioRxiv·Dec 22, 2020
- Transcriptomically-guided mesendoderm induction of human pluripotent stem cells using a systematically defined culture scheme
R. L. Carpenedo, S. Y. Kwon, … W. L. Stanford·bioRxiv·Feb 26, 2019
- Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA
K. Wang, R.-Z. Lin, … J. Melero-Martin·bioRxiv·Mar 2, 2020
- Direct induction of hemogenic endothelial progenitors from hPSCs by defined factors revealed by single-cell transcriptome analysis
L. N. Randolph, Y. Jiang, … X. L. Lian·bioRxiv·Mar 18, 2021