bioRxivMolecular BiologyPreprint
Splicing factor SRSF1 expands the regulatory logic of microRNA expression
Marija Dargyte, Julia PhilippiD, Christina Palka, Michael D. StoneiD, Jeremy R. SanfordiD
Abstract
The serine and arginine-rich splicing factor SRSF1 is an evolutionarily conserved, essential pre-mRNA splicing factor. Through a global protein-RNA interaction survey we discovered SRSF1 binding sites 25-50nt upstream from hundreds of pre-miRNAs. Using primary miRNA-10b as a model we demonstrate that SRSF1 directly regulates microRNA biogenesis both in vitro and in vivo. Selective 2 hydroxyl acylation analyzed by primer extension (SHAPE) defined a structured RNA element located upstream of the precursor miRNA-10b stem loop. Our data support a model where SRSF1 promotes initial steps of microRNA biogenesis by relieving the repressive effects of cis-regulatory elements within the leader sequence.
- Citations
- 3
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Controlled by disorder: phosphorylation modulates SRSF1 domain availability for spliceosome maturation
Talia Fargason, Erin Powell, … Jun Zhang·Protein SciencePeer-reviewed·Feb 19, 2025
- SRSF12 is a primate-specific splicing factor that induces a tissue-specific gene expression program
Jimmy Ly, Sarah L. Cady, … Iain M. Cheeseman·Molecular Biology of the CellPeer-reviewed·Oct 8, 2025
- Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis
- The DEAD-box helicase RCF1 plays roles in miRNA biogenesis and RNA splicing in Arabidopsis
- miR-1/206 down-regulates splicing factor Srsf9 to promote myogenesis
K. K. Bjorkman, M. Buvoli, … L. A. Leinwand·bioRxiv·Jul 3, 2019
- Dual function of ERH in primary miRNA biogenesis
Simon Aschenwald, Aswini Kumar Panda, … Sebastian Herzog·Nucleic Acids ResearchPeer-reviewed·Jun 2, 2026