Iain M. Cheeseman

The paper identifies the proteasome's role in regulating CENP-T levels to prevent non-centromere kinetochore assembly.

The paper shows that mammalian cells produce extended HP1β isoforms that alter effector binding preferences, affecting heterochromatin organization.

This study identifies gene targets involved in cell-substrate adhesion through functional genetic analyses in human cells, revealing dynamic requirements and novel regulators.

This study develops a machine learning model to predict protein stability from N-terminal sequences and identifies stability-determining motifs in annotated and alternative isoforms.

Brieflow is a computational pipeline for analyzing optical pooled screening data, demonstrating its ability to recover biological modules missed by existing methods.

The paper introduces OttoSeq, an automated optical pooled screen platform that completes a genome-wide cell painting screen in eight days, analyzing over 5 million cells across 21,732 gene knockouts.

The paper examines centromere protein dynamics during quiescence entry and exit, showing rapid disassembly and reassembly of centromere proteins with specific timing for CENP-A deposition.

This study shows that 5' UTR length influences alternative N-terminal protein isoform production through start codon selection and demonstrates this in health and disease.

This study identifies SRSF12 as a primate-specific splicing factor that induces tissue-specific gene expression programs in human cells.

The paper identifies KIF18A as a gene whose knockout causes synthetic lethality with a hypomorphic CENP-C mutant, revealing a regulatory system for chromosome congression.

The paper shows that during prolonged mitotic arrest, the transcriptome is stabilized through increased mRNA half-lives and reduced deadenylation, dependent on PABPC1 and PABPC4.

This study investigates the role of the SAC in mitotic timing and error surveillance, finding that Mad2 is dispensable for viability in Cdc20 {Delta}FL cells and that mitotic timing depends on multiple interdependent pathways.

The paper identifies differentially-localized protein isoforms generated by alternative start codon selection and shows their role in mitochondrial function, evolution, and disease.

The paper identifies C18orf21/RMRPP1 as an RNase MRP-specific protein, reveals its role in 40S ribosome biogenesis, and identifies Nepro as an essential rRNA processing factor.

The paper shows that loss of 19S proteasome subunits causes monopolar spindles by ubiquitin-independent degradation of KIF11, highlighting a non-canonical role for the proteasome in mitosis.

The paper shows that Ndc80 binding to CENP-T is enhanced by molecular density, with maturation of binding sites in clusters accelerating complex formation.

The paper identifies gene targets that modulate sensitivity to anti-microtubule drugs using CRISPR screens in human cells.

The paper shows that eIF1 release during mitosis increases start-codon selection stringency, altering translation and affecting mitotic cell survival.

This study identifies a germline-specific DSN1 splice isoform essential for oocyte maturation and early embryonic development in mammals.

The paper investigates the evolutionary dynamics of the kinetochore complex across 70 mammalian species, finding that structural proteins evolve rapidly while regulatory genes are conserved.

The paper identifies PRR12 as a cohesin regulator through co-essentiality analysis, showing its role in genome integrity and context-specific effects across species.