High-quality proteins and RNAs extracted from exact same samples for proteomics and RNA-Seq analyses

Back to the 1990 the single step method developed by Chomczynski and Sacchi for RNA isolation was extended for sequential isolation of RNA, DNA and proteins from a same sample. Although the quality of the extracted RNA turned compatible with RNA-Seq analyses, the extraction of the protein fraction from the same sample was time-consuming and resulting in low yield and quality of proteins not compatible with LC-MS proteomic analyses. Here we report a novel procedure by isolating in parallel the protein fraction and the RNA fraction from the same exact minute mosquito samples. We provide evidence that each cognate fractions are compatible with LC-MS proteomic analysis on the one hand and RNA-Seq analysis on the other hand. This protocol is simple, time efficient and adequate for studies involving limited sample size and could be applied easily to a broad range of animal and human samples.