hnRNP R is a regulator of stress granule formation

Abstract

hnRNP R (Heterogeneous nuclear ribonucleoproteins R) is one member of RNA-binding proteins (RBPs) in hnRNP family, it plays important role in nucleic acid metabolism including alternative splicing, mRNA stabilization, transcriptional and translational regulation, and mRNA transport. Mostly hnRNP R is localized in nuclear and extensively binds with RNA and chromatin, but still some part of it exports to cytosol. We found that long and short isoform hnRNP R can located in stress granules, depletion hnRNP R can facilitate the stress granule formation and accelerate mRNA in the stress granules. Meanwhile we found that depletion hnRNP R can alter the stress granule phenotype and impair stress granule resolving. Additionally, we also found that depletion hnRNP R also induce TDP43 granules formation. Furthermore, depletion hnRNP R can impair TDP43 binding with ribosome. Altogether, hnRNP R plays important role in the stress granule formation.